Secretion of human interleukin 2 by recombinant Mycobacterium bovis BCG

Depletion of Endogenous Interleukin-10 Augments Interleukin-1b Secretion by Mycobacterium bovis BCG-Reactive Human Cells

Recombinant Mycobacterium bovis BCG secreting functional interleukin-2 enhances gamma interferon production by splenocytes.

Mycobacterium bovis BCG was genetically engineered to express and secrete mouse interleukin-2 (IL-2) and rat IL-2. Genes encoding IL-2 were inserted into an Escherichia coli-BCG shuttle plasmid under the control of the BCG HSP60 promoter. To facilitate study of proteins produced in this system, the IL-2 gene product was expressed (i) alone, (ii) with the mycobacterial alpha-antigen secretion si...

Depletion of endogenous interleukin-10 augments interleukin-1 beta secretion by Mycobacterium bovis BCG-reactive human cells.

In this study, we found evidence that the interleukin-10 (IL-10) protein is functionally relevant in Mycobacterium bovis BCG-induced cytokine synthesis, as neutralization of endogenously synthesized IL-10 in human cells activated with BCG resulted in a two- to threefold increase in the level of IL-1 beta. When exogenous recombinant human IL-10 was added to human mononuclear cells, a significant...

Differentiation between Mycobacterium bovis BCG-vaccinated and M. bovis-infected cattle by using recombinant mycobacterial antigens.

Tuberculosis continues to be a worldwide problem for both humans and animals. The development of tests to differentiate between infection with Mycobacterium tuberculosis or Mycobacterium bovis and vaccination with M. bovis BCG could greatly assist in the diagnosis of early infection as well as enhance the use of tuberculosis vaccines on a wider scale. Recombinant forms of four major secreted pr...

A double recombinant Mycobacterium bovis BCG strain for screening

12 Affiliations: 13 14 Vandana Singh 15 Senior Project Fellow 16 Division of Microbiology 17 CSIR-Central Drug research Institute 18 Lucknow, 226031, INDIA, E. mail: [email protected] 19 20 Rajesh Kumar Biswas 21 Senior Research Fellow 22 Division of Microbiology 23 CSIR-Central Drug research Institute 24 Lucknow, 226031, INDIA, E. mail: [email protected] 25 26 27 28 29 Corresponden...